Creutzig U, Harbott J, Sperling C, Ritter J, Zimmermann M, Lffler H, Riehm H, Schellong G, Ludwig WD. PMC Discussion. Usually, 20 mL of pleural or peritoneal fluid is sufficient. . (2018 March 12). The prognostic value of immunophenotyping in AML is controversial [ 3]. Additional FISH or molecular testing may be recommended by the Mayo pathologist to facilitate diagnosis. Medscape Hematology. Unable to load your collection due to an error, Unable to load your delegates due to an error. However, treatment with chemotherapy may eliminate the abnormal cells, and if treatment is successful, normal white blood cells (WBCs) will replace abnormal cells. It is not offered in every laboratory, but many larger hospitals and academic medical centers perform the testing or your sample may be sent to a reference laboratory. She always had a keen interest in medical and health science. Large granular lymphocytic leukemia: a brief review. For assistance, contact. -Bone Marrow Staging for Known or Suspected Malignant Lymphoma Algorithm, -Acute Myeloid Leukemia: Testing Algorithm, -Acute Myeloid Leukemia: Relapsed with Previous Remission Testing Algorithm, -Acute Promyelocytic Leukemia: Guideline to Diagnosis and Follow-up, -Mast Cell Disorder: Diagnostic Algorithm, Bone Marrow, -Acute Leukemias of Ambiguous Lineage Testing Algorithm, Acute Leukemia -- Immunophenotyping, Flow Cytometry, Chronic Lymphocytic Leukemia, Immunophenotyping, Flow Cytometry, Flow Cytometry, Leukemia Immunophenotyping, Flow Cytometry, Lymphoma Immunophenotyping, Lymphoma Immunophenotyping by Flow Cytometry, GLL Panel - Leukemia Immunophenotyping (ALWAYS order LCMS), Granular Lymphocytic Leukemia (ALWAYS order LCMS), KIR Panel - Leukemia Immunophenotyping (ALWAYS order LCMS), LGL Panel - Leukemia Immunophenotyping (ALWAYS order LCMS), NK Panel - Leukemia Immunophenotyping (ALWAYS order LCMS), B-cell ALL minimal residual disease (MRD) detection. This technique helps in prognostication and is also used to differentiate between neoplastic and reactive expansions of lymphocytes. Epub 2018 Aug 6. Reflex tests will be performed at an additional charge for each marker tested (FIRST if applicable, ADD1 if applicable). By junio 4, 2022 masonry pilaster details junio 4, 2022 masonry pilaster details This case suggested that chromosomal alterations may precede morphological, flow cytometric and clinical changes and accelerate progression of the disease. It's also used to diagnose and classify leukemia or lymphoma. "What is Immunophenotyping?". Do not aliquot. 3. We describe the clinicopathologic, cytogenetic, and molecular genetic characteristics of 14 cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with t(14;19)(q32;q13). A normal cell will display a pattern of antigens that correlates with the type and maturity of the cell. Maturation-associated immunophenotypic abnormalities in bone marrow Background: Atypical lymphocytosis is a common peripheral blood abnormality seen not only in Epstein-Barr virus (EBV)-associated acute infectious mononucleosis but also in other conditions, including other viral infections, cancer, immune . Jaffe, E. et. Anaplastic lymphoma kinase protein was detected in about 33% (3/9) of ALCLs examined by flow cytometric immunophenotyping (FCI); expression was validated by immunohistochemical analysis. This approach, called immunohistochemistry, is used every day for some leukemia and lymphoma markers and other types of cancer. Upper endoscopy revealed a neoplastic growth at . no immunophenotypic abnormalities detected, failed to save changes to sbc squad companion app. MeSH Of 19 . The testing process begins with a screening panel. Cancers (Basel). However, lymphoma cells may or may not find their way to the bloodstream and might require other collection techniques. TdT and PAX5 were performed in five of the seven patients with ABLB detected by FC. Flow cytometry immunophenotyping may be useful in helping to diagnose, classify, treat and determine prognosis of these blood cell cancers. In agreement with previous studies, no immunophenotypic features (other than monocytic differentiation) predicted the presence of an 11q23 rearrangement. Available online at https://www.nlm.nih.gov/medlineplus/ency/article/003518.htm. and transmitted securely. No evidence of ATM (11q22.3) deletion. Accessed April 2011. Normal granulocytes show sequential progression from promyelocytes . An ASCUS pap smear result is considered to be mildly abnormal. Constrictive Pericarditis-A Cloak Camouflaging Lymphoma Immunophenotyping detects the presence or absence of antigens found on the surface or interior of blood cells. "What is Immunophenotyping?". 1985 Aug 29;313(9):534-8 Front Oncol. 2004 Mar;121(3):373-383. doi: 10.1309/3A32-DTVM-H640-M2QA, 7. In general, these criteria involved identification of abnormal expression or loss of antigens in B- and T-lineage populations. Immunophenotypic diagnosis of non-Hodgkin's lymphoma in paraffin sections. Flow cytometric immunophenotyping is an established method for the detection of occult leptomeningeal disease in patients with aggressive B-cell non-Hodgkin lymphoma, and is increasingly being used in the evaluation of patients without an established diagnosis of lymphoma who present with signs and/or symptoms referable to the central nervous Accessibility Li Y, Wei J, Mao X, Gao Q, Liu L, Cheng P, Liu L, Zhang X, Zhang K, Wang J, Zhu L, Zhou J, Zhang Y, Meng L, Sun H, Li D, Huang M, Huang W, Deng J, Zhang D. PLoS One. Federal government websites often end in .gov or .mil. Tel19p/19q used to detect copy number abnormalities of chromosome 19, reveal a hybridization pattern within normal limits in 200 analyzed nuclei. Treatment of plasma cell neoplasms (including multiple myeloma, monoclonal gammopathy of undetermined significance, and plasmacytoma) includes observation, chemotherapy, radiation therapy, stem cell rescue, targeted therapy, immunotherapy, and supportive therapies. Immunophenotype is a key parameter that is very valuable in predicting response to treatment as well as survival rates. Am J Clin Pathol. Chronic lymphocytic leukemia is an extremely heterogeneous disease and prognostic factors such as chromosomal abnormalities are important predictors of time to first treatment and survival. 2023 TESTING.COM. while also discussing the various products Sartorius produces in order to aid in this. Leuk Res. Antibodies are made up of chains of protein : 2 long (heavy) chains and 2 shorter (light) chains. There is increasing evidence of T cell dysfunction in B cell chronic lymphocytic leukaemia (B-CLL) which may contribute to the aetiology and progress of the disease. A positive correlation was found between CD34+ and CD34 B-cell precursors (r . In fact, these two markers are not normally expressed together. (2008 December 1). This technique also helps identify or confirm the cell of origin in non-hematopoietic neoplasia. Immunologic monitoring in adults with acute lymphoblastic leukemia. By continuing to browse this site you agree to our use of cookies. Flow cytometry immunophenotyping may be ordered when you have an increased number of lymphocytes (or sometimes an increase in another type of white blood cell, WBC), anemia, a decreased platelet count, or immature WBCs that are not normally seen in the blood. Application of these criteria to a series of nearly 500 cases of lymphoma indicated that over 90% of B-lineage and about 80% of T-lineage neoplasms manifested immunophenotypic abnormalities that could distinguish them from benign, reactive lymphoid processes. These abnormalities were related to immunophenotypic markers as This study prospectively analysed the relationships between immunophenotypic and cytogenetic features of blast cells in 432 acute non-lymphoblastic leukemias (ANLL) at presentation. More info. Accessed December 2014. Jevremovic D, Dronca RS, Morice WG, et al: CD5+ B-cell lymphoproliferative disorders: Beyond chronic lymphocytic leukemia and mantle cell lymphoma. Tietz Clinical Guide to Laboratory Tests, 4th Edition: Saunders Elsevier, St. Louis, MO. While some antigens are found only on one type of cell, others are found on different types. Background Myeloid Sarcoma with monocytic differentiation is rare and quite likely is missed by surgical pathologists. Khalidi HS, Medeiros LJ, Chang KL, Brynes RK, Slovak ML, Arber DA. An absolute CD8+ lymphocytosis correlates with disease progression and low expression of CD4 and CD8 (as found in autoimmune disease) The study was aimed to investigate the immunophenotypic and cytogenetic features of chronic lymphocytic leukemia (CLL) in order to provide an evidence for diagnosis and therapy. Chronic lymphocytic leukemia. Overall, del(13q14) and +12 were the most common abnormalities (39%), whereas del(11q13), del(17p13), and del(6q23) were detected only in 3, 1, and 0 cases, respectively. MDS is distinguished from other disease processes by a pattern of multiple myeloid immunophenotypic abnormalities (3-6). FOIA Abnormal T-cell function in B-cell chronic lymphocytic leukaemia A stable aberrant immunophenotype characterizes nearly all cases of Leuk Res. If not ordering electronically, complete, print, and send 1 of the following forms with the specimen: Specimens will be initially triaged to determine which, if any, of. (PDF) Immunophenotypic Analysis of Anaplastic Large Cell - ResearchGate It has become a common technique for the identification and classification of acute leukemias, particularly acute myeloid leukemia (AML). official website and that any information you provide is encrypted although diagnostic criteria are well established, a no immunophenotypic myeloid abnormalities were detected in the healthy donor bone marrow aspirates or in the 10 remission bone marrow aspirates from patients with a history of nonmyeloid neoplasia table 3, as mentioned, the immunophenotypic panels used evolved during the study, and not all The translocation t(9;22)(q34;q11.2) was detected by conventional chromosomal analysis in 59 patients (91%) the Ph-positive ALL cohort. no immunophenotypic abnormalities detectedpower bi search multiple values Haziran 10, 2022 / community funeral home pikeville, ky obituaries / in walks from bowleaze cove / tarafndan Trisomy 12 is the second most frequent aberration detected by fluorescence in situ hybridization at the time of diagnosis (10-25%), and it confers an intermediate prognostic risk, with a median time to . I got thre results today, which were "no significant abnormalities". 1993 Mar;9(4-5):285-91. doi: 10.3109/10428199309148525. Flow cytometry immunophenotyping may be performed on blood, bone marrow, or other samples to provide this additional information. Flow lymphoma is used in the case of lymphoid neoplasms or when a lymphoid origin is suspected on the basis of cell morphology after staining. Acute Lymphoblastic Leukemia (ALL). PMC (2012 February 17). An official website of the United States government. National Cancer Institute [On-line information]. 3. Korean J Lab Med. Immunophenotyping is widely used to identify and classify AML. All rights reserved. Phenotypic analysis by flow cytometry of surface immunoglobulin light chains and B and T cell antigens in lymph nodes involved with non-Hodgkin's lymphoma. on this website is designed to support, not to replace the relationship Second, unusual expression of surface antigens in ANKL cells was a prominent feature. 2018 Jun 1;128(6):2519-2534. doi: 10.1172/JCI97053. According to the European Group for the Immunological Classification of Leukemias (EGIL), AML can be immunologically defined by the expression of atleast two of the following myeloid markers: Based on this classification, one study researched the prognostic significance of various immunophenotypic subgroups in 177 adult AML patients. Flow cytometric immunophenotyping for hematologic neoplasms. CD20 is a marker of maturity and CD34 is a marker of immaturity. Atypical cells don't necessarily mean you have cancer. What is Immunophenotyping? - News-Medical.net degree in Chemistry and Master of Science (M.Sc) degree in Biochemistry from the University of Calicut, India. Application of immunophenotypic analysis in distinguishing chronic myelomonocytic leukemia from reactive monocytosis. Clipboard, Search History, and several other advanced features are temporarily unavailable. This finding confirms the varied pathogenetic mechanisms leading to hemophagocytosis, and prompts further . no immunophenotypic abnormalities detected. (Reviewed 2013 July 10). Or it can be the result of a specific treatment. No flow cytometric abnormalities were detected in CD4-positive T-cells from 10 control patients without lymphoproliferative disorders. News-Medical. Chronic active Epstein-Barr virus infection progresses to aggressive NK cell leukemia with a poor prognosis. No significant associations were detected between the presence of flow cytometric abnormalities (defined as 2 or more abnormalities) in RCC patients and age or sex, the presence of human leukocyte antigen (HLA)-DR15 (found in an increased frequency in adult low-grade MDS and aplastic anemia patients 33 32 and associated with a better response to 8600 Rockville Pike Two or more immunophenotypic abnormalities were detected in 49 of 81 RCC patients (60%), and in 2 of 17 (v)SAA patients (12%). Specimens will be initially triaged to determine which, if any, of the immunophenotyping panels should be performed. In agreement with previous studies, no immunophenotypic features (other than monocytic differentiation) predicted the presence of an 11q23 rearrangement. (Reviewed 2010 December). News-Medical. gayle telfer stevens husband Order Supplement. TdT and PAX5 were performed in five of the seven patients with ABLB detected by FC. 1985 Aug 29;313(9):539-44 The lady explained that that meant I didn't have anything preconcerous, but she didn't see to know what it DID mean. Both mature and immature B cells are normally positive for the CD19 marker. Susha has a Bachelor of Science (B.Sc.) Seiter, K. (2018 July 17, Updated). http://www.cancer.gov/publications/dictionaries/cancer-terms?cdrid=341450, http://www.nature.com/leu/journal/v20/n7/full/2404242a.html, http://www.bloodjournal.org/content/96/3/870?sso-checked=true. Leukemia & Lymphoma Society [On-line information]. A correlation study of immunophenotypic, cytogenetic, and clinical features of 180 AML patients in China . Available online at https://www.cancer.gov/cancertopics/factsheet/detection/laboratory-tests. CD34 cells can be detected in cord blood, bone marrow and in the peripheral blood of normal subjects, where they constitute respectively about 1.5% and 0.1-0.01% of the elements . . Verbal Irony In Romeo And Juliet Act 2. Correlation assay showed that t(8;21) was only present in 16 AMLM2 patients, and strongly . We use cookies to enhance your experience. Mcclellan Oscillator Website, Flow Cytometric Immunophenotyping Is Sensitive for the Early Diagnosis of De Novo Aggressive Natural Killer Cell Leukemia (ANKL): A Multicenter Retrospective Analysis. Please enable it to take advantage of the complete set of features! Unit Code 3287: Leukemia/Lymphoma Immunophenotyping by Flow Cytometry. No significant immunophenotypic abnormality was detected by flow cytometry. Two atypical human non-Hodgkin's lymphomas (NHLs) that exhibited unusual genotypic and in situ immunophenotypic abnormalities are described. 2015 May;169(3):368-376. doi: 10.1111/bjh.13303, 5. Furthermore, abnormal T-cell populations can be detected by using a panel of antibodies; . Interestingly, some of the other antigens present might suggest a specific genetic subtype of B-lymphoblastic leukemia, which also might have a certain prognosis. If . Shi M, Ternus JA, Ketterling RP, et al: Immunophenotypic and laboratory features of t(11;14)(q13;q32)-positive plasma cell neoplasms. If cell count is less than 10 cells/mcL, a larger volume of spinal fluid may be required. In univariate analysis, CD9, CD10, CD15, CD34 and TdT expression appeared significantly associated with chromosomal anomalies. In the present study, we describe both quantitative and qualitative immunophenotypic abnormalities involving BM B-cells in MDS patients. These may be the first indication of a possible blood cell cancer. no diagnostic immunophenotypic abnormalities detected 2010 May;34(5):594-7. doi: 10.1016/j.leukres.2009.08.029. J Adv Pract Oncol. This test will be processed as a laboratory consultation. [Flow cytometric analysis of surface phenotypes in B-cell non-Hodgkin's lymphoma]. In this case report of a child with mosaic T21 and DS-AMKL, flow cytometry performed on BMA showed no immunophenotypic abnormalities, morphological review of BMA revealed no clusters of tumor cells, and BMA failed to show the expected GATA1 mutation. Accessibility Immunophenotypic identification of acute myeloid leukemia with - Nature Accessed April 2011. Understanding Lab and Imaging Tests. Immunophenotyping, a common application in flow cytometry, allows multiple cell surface markers to be simultaneously characterized on a per-cell basis.Immunophenotyping can be difficult by flow cytometry, however, when only a small number of cells are available. Therefore, the need to explore a new marker that can . These antigens are protein structures found on or within WBCs. This process is widely used to diagnose different types of lymphoma and leukemia by comparing normal cells and cancer cells. Originally, glass slides with fixed tissue sections were treated with an antibody that was specific for a type of antigen typically found on certain abnormal cells associated with a particular leukemia or lymphoma. no immunophenotypic abnormalities detected - bigbangblog.net It can detect normal cells as well as abnormal cells whose pattern of markers are typically seen with specific types of leukemia and lymphoma. In addition, reflex testing may occur to fully characterize a disease state or clarify any abnormalities from the screening test. Anders PM, Montgomery ND, Montgomery SA, Bhatt AP, Dittmer DP, Damania B. J Clin Invest. If no abnormalities are detected by the initial panel, no further flow cytometric assessment will be performed unless otherwise indicated by specific features of the clinical presentation or prior laboratory results. American Society for Clinical Pathology; 2007; Betters DM: Use of flow cytometry in clinical practice. Based on these findings, we provide an objective marker based on clinical data for the definite diagnosis of ANKL. BM: hematogones . For solid tissue specimens, order LLPT / Leukemia/Lymphoma Immunophenotyping, Flow Cytometry, Tissue. 2022 Apr;71(4):919-932. doi: 10.1007/s00262-021-03051-x. (+632) 7110427 | (+632) 7110383 Integrity Aesthetic Building, 788 Banawe Avenue, Quezon City, Philippines info@integrityaesthetic.ph JAMA Patient Page V301 (4) [On-line information]. Table 1. Flow cytometric analysis of the peripheral blood shows no immunophenotypic evidence for an abnormal B cell or T- cell population, and no circulating blasts. or negative if no abnormal population was detected. Accessed April 2011. Available online at https://arupconsult.com/content/acute-lymphoblastic-leukemia. Diverse immunophenotypic abnormalities were seen in patients with aHLH; the type of aberrant phenotype had no relationship to either clinical or laboratory findings, underlying/predisposing factors or to the response to treatment. What is Immunophenotyping?. MeSH Usually, 1 to 1.5 mL of spinal fluid is sufficient. If no abnormalities are detected by the initial panel, no further flow cytometric assessment will be performed unless otherwise indicated by specific features of the clinical presentation or prior laboratory results. Detection of Bcell populations with monotypic light chain expression In case 14, a patient had PCM with del(13q/RB1) as a sole abnormality detected by FISH and this patient's disease remained active during the following 17 months. Available online at https://www.cancer.org/acs/groups/cid/documents/webcontent/003109-pdf.pdf. 4th ed. Positive Ph status was the sole abnormality in 19 patients (32%) and was associated with other abnormalities in 43 patients (73%). Br J Haematol. The type of sample to be tested is up to your healthcare practitioner and must be representative of your cancer. The percentage and pattern of cells staining for CD34, TdT, and PAX5 . Flow cytometry may be used to characterize and count types of white blood cells in the evaluation of infectious diseases, autoimmune disorders or immunodeficiencies. Classification of MDS patients according to the patterns of expression of multiple. The immunophenotype of adult acute myeloid leukemia: high frequency of lymphoid antigen expression and comparison of immunophenotype, French-American-British classification, and karyotypic abnormalities. The above negative findings can be attributed to low leukemia burden in the BMA. Classification of lymphoid neoplasms: the microscope as a tool for disease discovery. Furthermore, in difficult cases or those with limited material or poor histology, immunophenotypic analysis may be the only means of making a definitive diagnosis. Stay up to date with the latest news and information from Testing.com by subscribing to our newsletter. Several studies have identified a relationship between AML prognosis and antigens such as CD7, CD9, CD11b, CD13, CD14, CD15, CD33, CD34, and CD56, though some other studies report conflicting results. MayoClinic [On-line information]. The synergistic proapoptotic effect of PARP-1 and HDAC inhibition in cutaneous T-cell lymphoma is mediated via Blimp-1. Epub 2012 Sep 20. Submit only 1 of the following specimens: Preferred: Yellow top (ACD solution A or B), Acceptable: Green top (sodium heparin) or lavender top (EDTA), Slides: If possible, include 5 to 10 unstained blood smears labeled with two unique identifiers. 2018 Aug;59(8):1913-1919. doi: 10.1080/10428194.2017.1410885, 6. Accessed December 2014. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). 2022 Feb 15;12(1):17-32. eCollection 2022. Cuneo A, Ferrant A, Michaux JL, Boogaerts M, Demuynck H, Bosly A, Doyen C, Carli MG, Piva N, Castoldi G, et al. Available online at https://www.questdiagnostics.com/hcp/intguide/jsp/showintguidepage.jsp?fn=TG_Lymphoid_Neoplasms.htm. Available online at https://bloodjournal.hematologylibrary.org/content/111/8/3941.full. (Blood cells normally mature in the bone marrow and are released into circulation when they are mature or nearly mature.) An interpretation of the immunophenotypic findings and correlation with the morphologic features will be provided by a hematopathologist for every case. The overall incidence of different immunophenotypic aberrancies among the 44 MF/SS patients is summarized in Table 1. . Available online at https://www.nccn.org/professionals/physician_gls/pdf/all.pdf. An internal organ may or may not be a little bigger or a little smaller than normal but this is insignificant and no cause for worry. As part of her masters degree, she specialized in Biochemistry, with an emphasis on Microbiology, Physiology, Biotechnology, and Nutrition. sharing sensitive information, make sure youre on a federal 1985 May;134(5):2995-3002 A pathologist, often one specializing in the study of blood diseases and/or blood cell cancers (a hematopathologist), will consider the results from the complete blood count (CBC), differential, blood smear, bone marrow findings, and flow cytometry immunophenotyping as well as other tests in order to provide a diagnostic interpretation. Immunophenotyping by Flow Cytometry - Testing.com Earlier studies demonstrated that flow cytometric abnormalities are detected in multiple lineages (3-6) and correlate with morphology and cytogenetics (4,6). How To Create Google Form Link In Mobile, If not ordering electronically, complete, print, and send 1 of the following forms with the specimen: -Hematopathology/Cytogenetics Test Request (T726). The triage panel also includes antibodies to assess the number of CD3-positive T cells and CD16-positive/CD3-negative natural killer (NK) cells present. Leukemia & Lymphoma Society. Percentage of abnormal cells :91% B-cells, small size cells. Flow cytometric immunophenotyping is of great value to diagnosis of natural killer cell neoplasms involving bone marrow and peripheral blood. Pp 1633-1711. Szary syndrome with multiple immunophenotypic aberrancies in tumor cells. Salaire De Naby Keita 2021, Kruglov O, Johnson LDS, Minic A, Jordan K, Uger RA, Wong M, Sievers EL, Shou Y, Akilov OE. I just had a colposcopy done to follow up on an ASCUS pap with high risk HPV. Blood Tests. Our results present evidences of an abnormal B-cell maturation in MDS. All Rights Reserved. (+632) 7110427 | (+632) 7110383 B-cell leukemia/lymphoma panel. To help diagnose and classify a leukemia or lymphoma; to help guide treatment; to aid in determining prognosis; to detect and evaluate leukemia or lymphoma cells that remain after treatment or at disease relapse, When you have signs and symptoms that a health care practitioner thinks may be due to leukemia or lymphoma; to help classify the type of leukemia or lymphoma, identify treatment options, and predict the likely course of the disease; to evaluate whether treatment has been effective or detect disease that remains or comes back after treatment (relapse or recurrence). 2022 Aug 12;13:970183. doi: 10.3389/fimmu.2022.970183. The Global Landscape of EBV-Associated Tumors. Clinical review on features and cytogenetic patterns in adult acute myeloid leukemia with lymphoid markers. This site complies with the HONcode standard for trustworthy health information: verify here. This case suggested that chromosomal alterations may precede morphological, flow cytometric and clinical changes and accelerate progression of the disease. no immunophenotypic abnormalities detected Please note that medical information found Second, unusual expression of surface antigens in ANKL cells was a prominent feature. CD numbers represent a naming convention that is based on international consensus. Accessed January 2020. NCI CPTC Antibody Characterization Program. These plasma cells are negative for CD19. In this article, News-Medical talks to Sartorius about biosensing and bioprocessing in gene therapy, doi: 10.1371/journal.pone.0158827. Available online through https://www.lls.org. Tissue flow cytometry immunophenotyping in the diagnosis and Accessed January 2020. Bookshelf Genomic and immunophenotypic landscape of aggressive NK-cell leukemia. Leuk Lymphoma. al. . Accessed January 2020. Immunophenotyping hematopoietic specimens can help resolve many differential diagnostic problems posed by the clinical or morphologic features. no immunophenotypic abnormalities detected 1. You may have (or lack) certain antigens that are typically seen, yet you may still be diagnosed with a specific type of leukemia or lymphoma. Immunophenotyping is widely used for the following reasons: Two types of tests are used in immunophenotyping: The choice of test is based on the type of sample: Heres a brief overview of the two types of test methods: In flow cytometry, the sample may range from blood, fluids in the body cavity (such as peritoneal or pleural fluids), bone marrow, or solid tissues in liquid media.